DNA
SapI

Part:BBa_B0102:Design

Designed by: Heather Keller   Group: T7.2   (2007-03-05)


SapI restriction enzyme site (offset cutter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1


Design Notes

This part was designed for use with Heather Keller's alternative assembly method for blunt cloning RBS sequences upstream of a coding sequence.


Source

NEB Catalog, SapI is part #R0569S.

References